Holtby (knee) will not be available for Saturday's road game against the Canadiens, Isabelle Khurshudyan of The Washington Post reports.
Holtby set aside 27 of 29 shots for a Stadium Series win over the Maple Leafs in Annapolis, Maryland on Saturday.
Holtby will rough up the home crease Friday against the Maple Leafs.
Holtby lasted only one period Monday, getting replaced by Philipp Grubauer after stopping 12 of 16 shots in a 5-1 loss to the Blue Jackets.
Holtby is expected to be the road starter against the Blue Jackets on Monday, Isabelle Khurshudyan of the Washington Post reports.
Holtby turned aside 30 of 33 shots during Thursday's 3-2 loss to the Panthers.
Holtby will guard the visiting goal Thursday in a road matchup with the Panthers, The Washington Post reports.
Holtby allowed four goals on just 19 shots during Tuesday's 4-2 loss to Tampa Bay.
Holtby was pulled from the crease after allowing six goals on 33 shots during Saturday's 7-1 loss to the Blackhawks.
Holtby will start in goal Saturday against the Blackhawks in Chicago, Isabelle Khurshudyan of The Washington Post reports.